Science & tech · 1992
Does exposing mouse eggs to visible light before IVF affect their development or implantation?
Compared with light-protected controls, mature mouse eggs exposed to 4,000 lux of visible light for 1, 2, or 4 hours before IVF showed no significant change in speed or rate. After blastocyst transfer, implantation rates and the proportion of normal fetuses were not found to differ in relation to the earlier light exposure.
Details
In a controlled experiment, mature mouse oocytes were exposed to visible light at an intensity of 4,000 lux for 1, 2, or 4 hours before in vitro fertilization (IVF). Control oocytes were cultured under identical conditions but protected from light.
Compared with these controls, the light-exposed eggs showed no significant change in cleavage speed or rate.
Blastocysts obtained through in vitro fertilization and culture were then transferred into the uteri of pseudopregnant females. Neither the implantation rate nor the proportion of normal fetuses was found to differ in relation to the oocytes’ earlier light exposure.
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